Applications of artificial intelligence (AI) in drinking water treatment processes: Possibilities.

In water treatment processes (WTPs), artificial intelligence (AI) based techniques, particularly machine learning (ML) models have been increasingly applied in decision-making activities, process control and optimization, and cost management. At least 91 peer-reviewed articles published since 1997 reported the application of AI techniques to coagulation/flocculation (41), membrane filtration (21), disinfection byproducts (DBPs) formation (13), adsorption (16) and other operational management in WTPs. In this paper, these publications were reviewed with the goal of assessing the development and applications of AI techniques in WTPs and determining their limitations and areas for improvement. The applications of the AI techniques have improved the predictive capabilities of coagulant dosages, membrane flux, rejection and fouling, disinfection byproducts (DBPs) formation and pollutants' removal for the WTPs. The deep learning (DL) technology showed excellent extraction capabilities for features and data mining ability, which can develop an image recognition-based DL framework to establish the relationship among the shapes of flocs and dosages of coagulant. Further, the hybrid techniques (e.g., combination of regression and AI; physical/kinetics and AI) have shown better predictive performances. The future research directions to achieve better control for WTPs through improving these techniques were also emphasized.

Insight into the chemical transformation and organic release of polyurethane microplastics during chlorination.

The ubiquitous occurrence of microplastics in water and wastewater is a growing concern. In this study, the chemical transformation and organic release of virgin and UV-aged thermoplastic polyurethane (TPU) polymers during chlorination were investigated. As compared to virgin TPU polymer, the UV-aged TPU polymer exhibited high chlorine reactivity with noticeable destruction on its surface functional groups after chlorination, which could be ascribed to the UV-induced activation of hard segment of TPU backbone and increased contact area. The concentrations of leached organics increased by 1.6-fold with obviously high abundances of low-molecular-weight components. Additives, monomers, compounds relating to TPU chain extension, and their chlorination byproducts contributed to the increased organic release. Meanwhile, the formation of chloroform, haloacetic acids, trichloroacetaldehyde, and dichloroacetonitrile increased by 3.8-, 1.7-, 4.9-, and 2.4-fold, respectively. Two additives and six chlorination byproducts in leachate from chlorinated UV-aged TPU were predicted as highly toxic, e.g., butyl octyl phthalate, palmitic acid, 2,6-di-tert-butyl-1,4-benzoquinone, and chlorinated aniline. Evaluated by human hepatocarcinoma cells, the 50% lethal concentration factor of organics released from chlorinated UV-aged TPU was approximately 10% of that from its virgin counterpart, indicating a substantially increased level of cytotoxicity. This study highlights that the release of additives and chlorination byproducts from the chemical transformation of UV-aged microplastics during chlorination may be of potentially toxic concern.

Comparative formation of chlorinated and brominated disinfection byproducts from chlorination and bromination of amino acids.

Amino acids are the main components of dissolved organic nitrogen in algal- and wastewater-impacted waters, which can react with chlorine to form toxic halogenated disinfection by-products (DBPs) in the disinfection process. In the presence of bromide, the reaction between amino acids and secondarily formed hypobromous acid can lead to the formation of brominated DBPs that are more toxic than chlorinated analogues. This study compares the formation of regulated and unregulated DBPs during chlorination and bromination of representative amino acids (AAs) (e.g., aspartic acid, asparagine, tryptophan, tyrosine, and histidine). In general, concentrations of brominated DBPs (trihalomethanes, haloacetonitriles, and haloacetamides, 24.9-5835.0 nM) during bromination were higher than their chlorinated analogues (9.3-3235.3 nM) during chlorination. This indicates the greater efficacy of bromine as a halogenating agent. However, the formation of chlorinated haloacetic acids during chlorination was higher than the corresponding brominated DBPs from bromination. It is likely that an oxidation pathway is required for the formation of haloacetic acids and chlorine is a stronger oxidant than bromine. Moreover, chlorine forms higher levels of haloacetaldehydes (74.4-1077.8 nM) from amino acids than bromine (1.0-480.2 nM) owing to the instability of brominated species. The DBP formation yields depend on the types of functional groups in the side chain of AAs. Eight intermediates resulting from chlorination/bromination of tyrosine were identified by triple quadrupole mass spectrometer, including N-chlorinated/brominated tyrosine, 3-chloro/bromo-tyrosine, and 3,5-dichloro/dibromo-tyrosine. These findings provided new insights into the DBP formation during the chlorination of algal- and wastewater-impacted waters with elevated bromide.

Characteristics and Stability of Ozone Nanobubbles in Freshwater Conditions.

The characteristics and stability of ozone nanobubbles (NBs) were investigated for the first time under different preparation conditions and freshwater conditions (i.e., pH, natural organic matter [NOM], carbonate, calcium, and temperature) for an extended period. Two oxygen gas flow rates (4 and 1 L/min) used in ozone NB generation affected the characteristics and stability of ozone NBs. The ozone NBs generated at a high initial dissolved ozone (12.5 mg/L) concentration showed a much higher brightness during measurements than the ozone NBs generated at a low initial dissolved ozone concentration (1 mg/L). The former also exhibited a higher negative surface charge and higher stability in comparison to the latter. The stability and half-lives of ozone NBs followed the order of 3 mM Ca2+ < pH 3 < NOM with high specific ultraviolet absorbance at 254 nm (SUVA254 = 4.1 L/mg·m) < pH 7 < pH 9, while the effects of carbonate and temperature were insignificant. Ozone NBs were relatively stable in waters for a long period (e.g., ≥ 60 days) except for high hardness or low pH conditions. Higher levels of hydroxyl radicals were produced from ozone NB solutions as compared to conventional ozonation.

Photodegradation of halogenated organic disinfection by-products: Decomposition and reformation.

In this study, the photodegradation of 33 different DBPs (trihalomethanes, haloacetic acids, haloacetaldehydes, and haloacetonitriles) and TOX with low pressure UV light and the subsequent reformation of DBPs with chlorine and monochloramine were investigated. Results indicated that photodegradation followed the order of TOI > TOBr > TOCl, and treated surface water with low SUVA254 background did not impact the photodegradation of highly UV susceptible DBPs such as triiodomethane (TIM), diiodobromomethane (DIBM), tribromomethane (TBM). The mass balance results of chloride, bromide and iodide showed that the main photodegradation mechanism of TOBr and TOI was dehalogenation supported by halide releases (i.e., Cl-, Br- and/or I- ion). In addition, the photodegradation removal effect was higher, when brominated DBPs formation was high. Although low pressure UV light effectively removed halogenated organic DBPs, subsequent use of disinfectants (Cl2 and NH2Cl) reformed photodegraded DBPs, and the overall DBPs concentrations were increased, which suggested that the released Br- and I- ions will reform DBPs in distribution systems, with oxidants present or added (e.g., booster chlorination) in distribution systems. This study showed that although UV photodegradation will reduce halogenated organic DBPs in distribution systems, especially more toxic iodinated and brominated DBPs, it will be a more effective technology towards the end of the distribution system or a point of entry solution rather than in distribution system with post-disinfection and residence time.

Effects of organic additives on spectroscopic and molecular-level features of photo-induced dissolved organic matter from microplastics.

The environmental occurrence and impact of dissolved organic matter leached from microplastics (MP-DOM) has been the subject of increased research interest. Commercial plastics, which typically contain additives, are subject to natural weathering processes and can eventually lose their additives. However, the effects of organic additives in commercial microplastics (MPs) on the release of MP-DOM under UV irradiation remain poorly understood. In this study, four polymer MPs (polyethylene; PE, polypropylene; PP, polystyrene; PS, polyvinylchloride; PVC) and four commercial MPs, including a PE zip bag, a PP facial mask, a PVC sheet, Styrofoam, were subjected to leaching under UV irradiation, and the MP-DOM was characterized using Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and fluorescence excitation emission matrix-parallel factor analysis (EEM-PARAFAC). Although UV light promoted the leaching of MP-DOM from both MP groups, the amount released was more pronounced for the polymer MPs than for the commercial MPs. The commercial MP-DOM was characterized by a prominent protein/phenol-like component (C1), while a humic-like component (C2) prevailed in the polymer MPs. FT-ICR-MS identified a higher number of unique molecular formulas for the commercial than for the polymer MP-DOM. The unique molecular formulas of commercial MP-DOM included known organic additives and other breakdown products, while the polymer MP-DOM featured more pronounced unsaturated carbon structures in its identified unique formulas. Several molecular-level parameters showed significant correlations with fluorescence properties, such as CHO formulas (%) with C1 and condensed aromatic structure (CAS-like, %) with C2, suggesting the potential application of fluorescent components as an optical descriptor for the complex molecular-level composition. This study also revealed the possible high environmental reactivity of both polymer MPs and fully weathered plastics due to the unsaturated structures generated in sunlit environments.

Co-Occurrence of Bromine and Iodine Species in US Drinking Water Sources That Can Impact Disinfection Byproduct Formation.

Bromine and iodine species are precursors for forming disinfection byproducts in finished drinking waters. Our study incorporates spatial and temporal data to quantify concentrations of inorganic (bromide (Br-), iodide (I-), and iodate (IO3-)), organic, and total bromine (BrT) and iodine (IT) species from 286 drinking water sources and 7 wastewater effluents across the United States. Br- ranged from <5-7800 µg/L (median of 62 µg/L in surface water (SW) and 95 µg/L in groundwater (GW)). I- was detected in 41% of SW (1-72 µg/L, median = <1 µg/L) and 62% of GW (<1-250 µg/L, median = 3 µg/L) samples. The median Br-/I- ratio in SW and GW was 22 µg/µg and 16 µg/µg, respectively, in paired samples with detect Br- and I-. BrT existed primarily as Br-, while IT was present as I-, IO3-, and/or total organic iodine (TOI). Inorganic iodine species (I- and IO3-) were predominant in GW samples, accounting for 60-100% of IT; however, they contributed to only 20-50% of IT in SW samples. The unknown fraction of IT was attributed to TOI. In lakes, seasonal cycling of I-species was observed and was presumably due to algal productivity. Finally, Spearman Rank Correlation tests revealed a strong correlation between Br- and IT in SW (RBr-,IT = 0.83) following the log10 (Br-, µg/L) = 0.65 × log10 (IT, µg/L) - 0.17 relationship. Br- and I- in treated wastewater effluents (median Br- = 234 µg/L, median I- = 5 µg/L) were higher than drinking water sources.

UV aging of microplastic polymers promotes their chemical transformation and byproduct formation upon chlorination.

The presence and accumulation of microplastics (MPs) in water and wastewater is a growing concern. When released to the water bodies, microplastics can be subject to surface weathering due to ultraviolet (UV) exposure. In this study, the effects of UV aging of six MP polymers from three groups (e.g., polyamide, polyester, and polyolefin) on their chlorine reactivity, chemical transformation, and formation of disinfection byproducts (DBPs) were studied. Polyamide (e.g., polyamide 6) in both virgin and UV-aged forms showed significantly higher chlorine demands than other MP polymers (915.5-947.9 versus 7.0-21.1 µmol/g MP in 24 h), and polyolefins were relatively inert to chlorine. UV aging enhanced the destructions of functional groups of polyamide and polyester upon chlorination, promoting the chlorine demands and leaching of organics by up to 1.7- and 2.4-fold, respectively. Polymer monomer and oligomers of polyamide 6 and toxic or endocrine disrupting additives (e.g., dimethyl phthalate and butyl octyl phthalate) were identified in leachates from chlorinated MP polymers by mass spectrometry. Meanwhile, up to >10-fold increases in the yields of trihalomethane, haloacetic acid, haloacetaldehyde, haloacetonitrile, and haloacetamide were observed from 30-day UV-aged MP polymers as compared to their virgin counterparts. Overall, this study reveals that UV aging can promote the reactivity and chemical transformation of MP polymers during chlorination, especially for polyamide and polyester, increase the release of polymer monomers, oligomers, and additives, and aggravate the role of MP polymers as DBP precursors.

Tracing microplastic (MP)-derived dissolved organic matter in the infiltration of MP-contaminated sand system and its disinfection byproducts formation.

Microplastic (MP) pollution in soil/subsurface environments has been increasingly researched, given the uncertainties associated with the heterogeneous matrix of these systems. In this study, we tracked the spectroscopic signatures of MP-derived dissolved organic matter (MP-DOM) in infiltrated water from MP contaminated sandy subsurface systems and examined their potential to form trihalomethanes (THMs) and haloacetic acids (HAAs) by chlorination. Sand-packed columns with commercial MPs (expanded polystyrene and polyvinylchloride) on the upper layer were used as the model systems. Regardless of the plastic type, the addition of MPs resulted in a higher amount of DOM during infiltration compared with the clean sand system. This enhancement was more pronounced when the added MPs were UV-irradiated for 14 days. The infiltration was further characterized using FT-IR and fluorescence spectroscopy, which identified two fluorescent components (humic-like C1 and protein/phenol-like C2). Compared with pure MP-DOM, C1 was more predominant in sand infiltration than C2. Further studies have established that C2 may be more labile in terms of biodegradation and mineral adsorption that may occur within the sand column. However, both these environmental interferences were inadequate for entirely expanding the spectroscopic signatures of MP-DOM in sand infiltration. The infiltration also exhibited a higher potential in generating carbonaceous disinfection byproducts than natural groundwater and riverside bank filtrates. A significant correlation between the generated THMs and decreased C1 suggests the possibility of using humic-like components as optical precursors of carbonaceous DBPs in MP-contaminated subsurface systems. This study highlighted an overlooked contribution of MPs in terms of the infiltration of DOM levels in sandy subsurface systems and the potential environmental risk when used as drinking water sources.

Removal of halides from drinking water: technological achievements in the past ten years and research needs.

Disinfection is an essential process for drinking water supplies resulting in the formation of unintended disinfection by-products (DBPs), many of which are potentially toxic and are known as the possible or probable human carcinogens. As of now, 100+ DBPs were characterized while about 600+ others can be formed in the supply water. To protect the human health, many regulatory agencies have set the guideline values for several DBPs. Removal of halide ions and natural organic matter prior to disinfection is an important step to reduce DBPs, and the associated exposure and risks. To date, many publications have reported various methods for halide removal from drinking water. The most review about halide removal technologies, associated challenges, and future research needs was published in 2012. Since then, a number of studies have been published on different methods of halide removal techniques. This paper aims to review the state of research on halide removal techniques focusing on the development during the past 10 years (2012-2021). The techniques were clustered into six major groups: adsorption, ion exchange, coagulation, advanced oxidation, membrane separation, and combined techniques. The progress on these groups of technologies, their advantages, and limitations were examined, and the future research directions to produce the safe drinking water were identified.

Formation of regulated and unregulated disinfection byproducts during chlorination and chloramination: Roles of dissolved organic matter type, bromide, and iodide.

Algal blooms and wastewater effluents can introduce algal organic matter (AOM) and effluent organic matter (EfOM) into surface waters, respectively. In this study, the impact of bromide and iodide on the formation of halogenated disinfection byproducts (DBPs) during chlorination and chloramination from various types of dissolved organic matter (DOM, e.g., natural organic matter (NOM), AOM, and EfOM) were investigated based on the data collected from literature. In general, higher formation of trihalomethanes (THMs) and haloacetic acids (HAAs) was observed in NOM than AOM and EfOM, indicating high reactivities of phenolic moieties with both chlorine and monochloramine. The formation of haloacetaldehydes (HALs), haloacetonitriles (HANs) and haloacetamides (HAMs) was much lower than THMs and HAAs. Increasing initial bromide concentrations increased the formation of THMs, HAAs, HANs, and HAMs, but not HALs. Bromine substitution factor (BSF) values of DBPs formed in chlorination decreased as specific ultraviolet absorbance (SUVA) increased. AOM favored the formation of iodinated THMs (I-THMs) during chloramination using preformed chloramines and chlorination-chloramination processes. Increasing prechlorination time can reduce the I-THM concentrations because of the conversion of iodide to iodate, but this increased the formation of chlorinated and brominated DBPs. In an analogous way, iodine substitution factor (ISF) values of I-THMs formed in chloramination decreased as SUVA values of DOM increased. Compared to chlorination, the formation of noniodinated DBPs is low in chloramination.

Effect of activated sludge treatment on the formation of Nnitrosamines under different chloramination conditions.

Municipal wastewater discharge is considered as one of the main sources of N-nitrosamine precursors which can impact the qualities of downstream source waters and reclaimed wastewaters for potable reuse. NNitrosamine precursors can be removed to various degrees during biological wastewater treatment (e.g., the activated sludge (AS) process). So far, little is known about the impact of the AS process on N-nitrosamine formation under practical disinfection condition (e.g., uniform formation condition (UFC)). In this study, N-nitrosamine UFC from selected model compounds, sewage components (i.e., blackwaters and greywaters) and sewage samples were comprehensively investigated during batch AS treatment tests. NNitrosodimethylamine (NDMA) formation from the tested precursor compounds (i.e., trimethylamine (TMA) and sumatriptan (SMTR)) under UFC chloramination decreased mostly after 6 or 24 hr treatment with different types of AS (i.e., domestic rural AS, domestic urban AS, and textile AS), and the reductions in NDMA UFC were comparable to their NDMA formation potential (FP) reductions. In urine and feces blackwaters, NDMA UFC increased after 6 or 24 hr treatment with the domestic (i.e., rural and urban) AS, while NDMA FP decreased substantially. The increases in NDMA UFC after AS treatment was presumably attributed to the removal of bulk organic matters (e.g., dissolved organic carbon (DOC)) which favored NDMA formation under UFC. On the other hand, in laundry greywaters having relatively abundant DOC, N-nitrosamine UFC was less affected by DOC removal before or after AS treatment, but decreased to similar degrees with N-nitrosamine FP. In sewage samples collected from wastewater treatment plants, N-nitrosamines UFC tended to increase or remain constant during AS treatment, despite the decreases in their FPs. These results suggest that biological wastewater treatment (e.g., the AS process) may not effectively reduce N-nitrosamine formation (e.g., measured under UFC) partially because the concurrent removal of bulk organic matters (e.g., DOC) favored N-nitrosamine formation in s econdary effluents.

Inputs of disinfection by-products to the marine environment from various industrial activities: Comparison to natural production.

Oxidative treatment of seawater in coastal and shipboard installations is applied to control biofouling and/or minimize the input of noxious or invasive species into the marine environment. This treatment allows a safe and efficient operation of industrial installations and helps to protect human health from infectious diseases and to maintain the biodiversity in the marine environment. On the downside, the application of chemical oxidants generates undesired organic compounds, so-called disinfection by-products (DBPs), which are discharged into the marine environment. This article provides an overview on sources and quantities of DBP inputs, which could serve as basis for hazard analysis for the marine environment, human health and the atmosphere. During oxidation of marine water, mainly brominated DBPs are generated with bromoform (CHBr3) being the major DBP. CHBr3 has been used as an indicator to compare inputs from different sources. Total global annual volumes of treated seawater inputs resulting from cooling processes of coastal power stations, from desalination plants and from ballast water treatment in ships are estimated to be 470-800 × 109 m3, 46 × 109 m3 and 3.5 × 109 m3, respectively. Overall, the total estimated anthropogenic bromoform production and discharge adds up to 13.5-21.8 × 106 kg/a (kg per year) with contributions of 11.8-20.1 × 106 kg/a from cooling water treatment, 0.89 × 106 kg/a from desalination and 0.86 × 106 kg/a from ballast water treatment. This equals approximately 2-6% of the natural bromoform emissions from marine water, which is estimated to be 385-870 × 106 kg/a.

The elimination of cell-associated and non-cell-associated antibiotic resistance genes during membrane filtration processes: A review.

With increasing water reuse as a sustainable water management strategy, antibiotic resistance genes (ARGs) which have been identified as emerging contaminants in wastewater are attracting global attentions. Given that wastewater treatment plants are now well-established as a sink and source of ARGs in both cell-associated and non-cell-associated forms, a need is acknowledged to reduce their proliferation and protect public health. Due to their different characteristics, cell-associated and non-cell-associated ARGs may have distinct responses to membrane filtration processes which are widely used as advanced treatment to the secondary effluent. This review improves the understanding of the abundance of cell-associated and non-cell-associated ARGs in wastewaters and the secondary effluents and compares the elimination of ARGs in cell-associated and non-cell-associated forms by low-pressure and high-pressure membrane filtration processes. The former process reduces the concentration of cell-associated ARGs by more than 2-logs on average. An increase of the retention efficiency of non-cell-associated ARGs is observed with decreasing molecular weight cut-offs in ultrafiltration. The high-pressure membrane filtration (i.e., nanofiltration and reverse osmosis) can effectively eliminate both cell-associated and non-cell-associated ARGs, with averagely more than 4.6-log reduction. In general, the two forms of ARGs can be removed from water by the membrane filtration processes via the effects of size exclusion, adsorption, and electrostatic repulsion. The size and conformation of cell-associated and non-cell-associated ARGs, characteristics of membranes, coexisting substances, and biofilm formation influence ARG retention. Accumulation and potential proliferation of cell-associated and non-cell-associated ARGs in foulants and concentrate and corresponding control strategies warrant future research.

Emerging investigator series: microplastic sources, fate, toxicity, detection, and interactions with micropollutants in aquatic ecosystems - a review of reviews.

Hundreds of review studies have been published focusing on microplastics (MPs) and their environmental impacts. With the microbiota colonization of MPs being firmly established, MPs became an important carrier for contaminants to step inside the food web all the way up to humans. Thus, the continuous feed of MPs into the ecosystem has sparked a multitude of scientific concerns about their toxicity, characterization, and interactions with microorganisms and other contaminants. The reports of common subthemes have agreed about many findings and research gaps but also showed contradictions about others. To unravel these equivocal conflicts, we herein compile all the major findings and analyze the paramount discrepancies among these review papers. Furthermore, we systematically reviewed all the highlights, research gaps, concerns, and future needs. The covered focus areas of MPs' literature include the sources, occurrence, fate, existence, and removal in wastewater treatment plants (WWTPs), toxicity, interaction with microbiota, sampling, characterization, data quality, and interaction with other co-contaminants. This study reveals that many mechanisms of MPs' behavior in aquatic environments like degradation and interaction with microbiota are yet to be comprehended. Furthermore, we emphasize the critical need to standardize methods and parameters for MP characterization to improve the comparability and reproducibility of the incoming research.

Predicting COVID-19 Infected Individuals in a Defined Population from Wastewater RNA Data.

Wastewater surveillance of SARS-CoV-2 RNA has become an important tool for tracking the presence of the virus and serving as an early indicator for the onset of rapid transmission. Nevertheless, wastewater data are still not commonly used to predict the number of infected individuals in a sewershed. The main objective of this study was to calibrate a susceptible-exposed-infectious-recovered (SEIR) model using RNA copy rates in sewage (i.e., gene copies per liter times flow rate) and the number of SARS-CoV-2 saliva-test-positive infected individuals in a university student population that was subject to repeated weekly testing during the Spring 2021 semester. A strong correlation was observed between the RNA copy rates and the number of infected individuals. The parameter in the SEIR model that had the largest impact on calibration was the maximum shedding rate, resulting in a mean value of 7.72 log10 genome copies per gram of feces. Regressing the saliva-test-positive infected individuals on predictions from the SEIR model based on the RNA copy rates yielded a slope of 0.87 (SE=0.11), which is statistically consistent with a 1:1 relationship between the two. These findings demonstrate that wastewater surveillance of SARS-CoV-2 can be used to estimate the number of infected individuals in a sewershed.

Preferential Halogenation of Algal Organic Matter by Iodine over Chlorine and Bromine: Formation of Disinfection Byproducts and Correlation with Toxicity of Disinfected Waters.

The increasing occurrence of harmful algal blooms (HABs) in surface waters may increase the input of algal organic matter (AOM) in drinking water. The formation of halogenated disinfection byproducts (DBPs) during combined chlorination and chloramination of AOM and natural organic matter (NOM) in the presence of bromide and iodide and haloform formation during halogenation of model compounds were studied. Results indicated that haloform/halogen consumption ratios of halogens reacting with amino acids (representing proteins present in AOM) follow the order iodine > bromine > chlorine, with ratios for iodine generally 1-2 orders of magnitude greater than those for chlorine (0.19-2.83 vs 0.01-0.16%). This indicates that iodine is a better halogenating agent than chlorine and bromine. In contrast, chlorine or bromine shows higher ratios for phenols (representing the phenolic structure of humic substances present in NOM). Consistent with these observations, chloramination of AOM extracted from Microcystis aeruginosa in the presence of iodide produced 3 times greater iodinated trihalomethanes than those from Suwannee River NOM isolate. Cytotoxicity and genotoxicity of disinfected algal-impacted waters evaluated by Chinese hamster ovary cell bioassays both follow the order chloramination > prechlorination-chloramination > chlorination. This trend is in contrast to additive toxicity calculations based on the concentrations of measured DBPs since some toxic iodinated DBPs were not identified and quantified, suggesting the necessity of experimentally analyzing the toxicity of disinfected waters. During seasonal HAB events, disinfection practices warrant optimization for iodide-enriched waters to reduce the toxicity of finished waters.

COVID-19 wastewater epidemiology: a model to estimate infected populations.

BACKGROUND: Wastewater-based epidemiology provides an opportunity for near real-time, cost-effective monitoring of community-level transmission of SARS-CoV-2. Detection of SARS-CoV-2 RNA in wastewater can identify the presence of COVID-19 in the community, but methods for estimating the numbers of infected individuals on the basis of wastewater RNA concentrations are inadequate. METHODS: This is a wastewater-based epidemiology study using wastewater samples that were collected weekly or twice a week from three sewersheds in South Carolina, USA, between either May 27 or June 16, 2020, and Aug 25, 2020, and tested for SARS-CoV-2 RNA. We developed a susceptible-exposed-infectious-recovered (SEIR) model based on the mass rate of SARS-CoV-2 RNA in the wastewater to predict the number of infected individuals, and have also provided a simplified equation to predict this. Model predictions were compared with the number of confirmed cases identified by the Department of Health and Environmental Control, South Carolina, USA, for the same time period and geographical area. FINDINGS: We plotted the model predictions for the relationship between mass rate of virus release and numbers of infected individuals, and we validated this prediction on the basis of estimated prevalence from individual testing. A simplified equation to estimate the number of infected individuals fell within the 95% confidence limits of the model. The rate of unreported COVID-19 cases, as estimated by the model, was approximately 11 times that of confirmed cases (ie, ratio of estimated infections for every confirmed case of 10·9, 95% CI 4·2-17·5). This rate aligned well with an independent estimate of 15 infections for every confirmed case in the US state of South Carolina. INTERPRETATION: The SEIR model provides a robust method to estimate the total number of infected individuals in a sewershed on the basis of the mass rate of RNA copies released per day. This approach overcomes some of the limitations associated with individual testing campaigns and thereby provides an additional tool that can be used to inform policy decisions. FUNDING: Clemson University, USA.

Chemical characterization of dissolved organic matter as disinfection byproduct precursors by UV/fluorescence and ESI FT-ICR MS after smoldering combustion of leaf needles and woody trunks of pine (Pinus jeffreyi).

Forested land plays an essential role in water supply across the United States (US). Smoldering commonly existing in wildfires contributes significantly to biomass consumption and gas emission, but its influence on source water quality has been rarely studied. Here, we investigated the impact of smoldering temperature (i.e., no burn, 250, 400, and 600 °C) on the nutrients, elements, and dissolved organic matter (DOM) of water extracts from the residues of the leaf needles and woody trunks of pine (Pinus jeffreyi) under the lab-simulated smoldering fire. Results showed the increase of pH and the yields of the dominated exchangeable cations of K+ and Mg2+, P, PO43--P, and SO42- with increasing temperature increasing from 250 to 600 °C, whereas significant decreases in the fraction of dissolved organic C in residue C with increasing temperature and the yields of dissolved organic carbon (DOC) and dissolved organic nitrogen (DON) after burnings. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) presented consistent results with UV/fluorescence, suggesting that the unburned materials contained more biodegradable tyrosine/tryptophan/soluble microbial byproduct-like compounds with high molecular weight (MW), whereas the 600 °C-smoldering materials composed of more aromatic, humified, fulvic/humic acid-like, and oxidized compounds with a potentially high density of C=C bonds had less reactivity in forming trihalomethanes (THMs) and haloacetonitriles (HANs). Our study indicates the smoldering-dominated prescribed fire as a potential forest management strategy for reducing biomass fuel and disinfection byproducts (DBPs) precursors in source water from forested lands.